中藥中的番瀉葉色譜分析sennosides in traditional chinese medicine
conditions
column: ace 3 c18-pfp
dimensions: 150 x 4.6 mm
part number: ace-1110-1546
mobile phase:
a: 0.75% acetic acid in h2o
b: mecn/meoh (90:10 v/v)
gradient:
time(mins)
%b
0
9
23
28
40
28
flow rate: 0.6 ml/min
temperature: 35 °c
detection: uv, 271 nm
sample: herbal tea bag containing folium sennae, peppermint,folium mori, folium nelumbinis,glycyrrhiza uralensis and lalang grass rhizome
analytes
sennoside a
sennoside b