成都摩爾科學(xué)儀器有限公司專(zhuān)業(yè)提供各種分析檢測(cè)專(zhuān)用色譜柱。色譜柱種類(lèi)齊全，分離度好,柱效高,重現(xiàn)性好，耐受性好，貨期短 ，產(chǎn)品線齊全，技術(shù)服務(wù)好，還有專(zhuān)業(yè)的技術(shù)人員一對(duì)一服務(wù)。下面給大家看看用成都摩爾科學(xué)儀器有限公司提供的srt sec分析專(zhuān)用色譜柱檢測(cè)分析的幾幅圖譜。heparin analysis on sec
column:srt sec-300 (7.8x300mm),srt sec-500 (7.8x300mm) andsrt sec-300 guard (4.6x50mm),column temperature: 30?c, flow rate: 0.6 ml/min, detection: ri, phase: 0.1 m ammoniumacetate, 0.02% sodium azide in water, injection volume: 20 µl of 5 mg/ml heparin
肝素檢測(cè)分析專(zhuān)用色譜柱
heparin analysis on sec and sax；
highlighted facts：
1：for heparin applications, sepax offer solutions for secseparations (under usp and ep conditions) as well asimpurity analyses by iex using sepax’s glycomix sax(also under usp conditions).
2：sepax’s sec columns come in a range of pore sizes togive the bestseparation of the mw range for thesetypes of samples.
3：we have shown that running two zenix sec-100 7.8x300mm columns can further increase the separationbetween mw species since resolution is correlated tocolumn length.
4：sepax’s glycomix sax was specifically developed toidentify any impurities in heparin samples, as shown byour separation between dermatan sulfate, an unknownimpurity, oversulfated chondroiton sulfate and heparin.
heparin analysis on sec and sax
lmw heparin-dalteparin and enoxaparin analysis onzenix ™ -100 (7830)
columns: zenix™-100 (3 ?m, 100 ?, 7.8x300 mm)
flow rate:
phase:
0.5 ml/min
2.84% na 2 so 4 , ph 5.0
temperature: 35 o c
detection: uv 234 nm and ri
injection volume: 25 µl
samples: 10 mg/ml dalteparin in water, ph 6.0, mw 3,000 – 8,000 d
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