nmt是基因功能的活體檢測(cè)技術(shù)，已被103位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用。
nmt歷*的今天
2014年02月19日，北京林業(yè)大學(xué)汪曉峰、李加國(guó)用nmt在planta 上發(fā)表了標(biāo)題為the fluxes of h2o2 and o2 can be used to evaluate seed germination and vigor of caragana korshinskii的研究成果。
期刊：planta
主題：h2o2和o2的通量可以評(píng)估種子的萌發(fā)和活力
標(biāo)題：the fluxes of h2o2 and o2 can be used to evaluate seed germination and vigor of caragana korshinskii
影響因子：3.347
檢測(cè)指標(biāo)：h2o2、o2流速
作者：北京林業(yè)大學(xué)汪曉峰、李加國(guó)
英文摘要
seed deterioration is detrimental to plant germplasm conservation, and predicting seed germination and vigor with reliability and sensitivity means is urgently needed for practical problems.
we investigated the link between hydrogen peroxide (h2o2) flux, oxygen influx and seed vigor of caragana korshinskii by the non-invasive micro-test technique (nmt). some related physiological and biochemical changes in seeds were also determined to further explain the changes in the molecular fluxes.
the results showed that there was a good linear relationship between germination and h2o2 flux, and that o2 influx was more suitable for assessing seed vigor. h2o2 flux changed relatively little initially, mainly affected by antioxidants (apx, cat and gsh) and h2o2 content; afterward, the efflux increased more and more rapidly due to high membrane permeability. with the damage of mitochondrial respiration and membrane integrity, o2 influx was gradually reduced.
we propose that monitoring h2o2 and o2 fluxes by nmt may be a reliable and sensitive method to evaluate seed germination and vigor.
中文摘要（谷歌機(jī)翻）
種子變質(zhì)對(duì)植物種質(zhì)的保存有害，因此迫切需要通過(guò)可靠性和敏感性手段預(yù)測(cè)種子的發(fā)芽和活力。
我們通過(guò)非損傷微測(cè)試技術(shù)（nmt）研究了過(guò)氧化氫（h2o2）通量，氧氣流入和檸條錦雞兒種子活力之間的聯(lián)系。還確定了種子中一些相關(guān)的生理和生化變化，以進(jìn)一步解釋分子通量的變化。
結(jié)果表明，發(fā)芽率與h2o2通量之間存在良好的線性關(guān)系，而o2流入更適合評(píng)估種子活力。h2o2通量初變化相對(duì)較小，主要受抗氧化劑（apx，cat和gsh）和h2o2含量的影響；之后，由于高的膜滲透性，流出量越來(lái)越快地增加。隨著線粒體呼吸和膜完整性的損害，o2流入量逐漸減少。
我們建議通過(guò)nmt監(jiān)測(cè)h2o2和o2通量可能是評(píng)估種子發(fā)芽和活力的可靠且敏感的方法。
fig. 3 effect of arti?cial aging on ?uxes of oxygen and h2o2. the colored lines represent the ?uxes of oxygen and h2o2 over time for one seed (a, b, d, e), and each column gives the mean ± sd from ten different seeds (n = 10) (c, f). statistical analysis was done using duncan’s multiple range test. treatments with different letters are signi?cantly different at the p < 0.05 level