nmt是基因功能的活體檢測(cè)技術(shù)，已被103位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用
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期刊：plant physiology
主題：aba充當(dāng)no3-傳導(dǎo)器激活tanrt2促no3-吸收
標(biāo)題：taanr1-tabg1 and tawabi5-tanrt2s/nars link aba metabolism and nitrate acquisition in wheat roots
影響因子：6.305
檢測(cè)指標(biāo)：no3-流速
檢測(cè)樣品：小麥根（分別距離根0.05、0.1、0.3、0.5、1.5、3、5、10、20、30 mm的位點(diǎn)）
no3-流實(shí)驗(yàn)處理方法：
15天的小麥幼苗，在0.2mm kno3/0.2mm kno3+50um aba處理1小時(shí)
no3-流實(shí)驗(yàn)測(cè)試液成份：未寫明
作者：中科院土壤所施衛(wèi)明、王萌，山東大學(xué)夏光敏
中文摘要（谷歌機(jī)翻）
硝酸鹽是大多數(shù)植物的氮素形式，既可作為營(yíng)養(yǎng)物又可作為信號(hào)分子。然而，控制面包小麥（世界上重要的農(nóng)作物之一）中硝酸鹽吸收的成分和調(diào)控因素仍不清楚，這主要是由于其六倍體基因組的復(fù)雜性。
在此，根據(jù)近發(fā)布的面包小麥全基因組信息，對(duì)高親和力硝酸鹽轉(zhuǎn)運(yùn)蛋白2（nrt2）和硝酸鹽同化相關(guān)（nar）基因家族進(jìn)行了表征。我們表明，硝酸鹽撤除后，硝酸鹽再供應(yīng)會(huì)刺激面包小麥根中的aba-ge解偶聯(lián)，從而導(dǎo)致根組織aba積累增強(qiáng)，而這種增強(qiáng)反過來又會(huì)影響根型nrt2 / nar基因的表達(dá)。顯示taanr1通過直接激活tabg1來調(diào)節(jié)硝酸鹽介導(dǎo)的aba積累，而tawabi5參與aba介導(dǎo)的nrt2 / nar基因的no3-誘導(dǎo)。
基于先前的證據(jù)，證明aba參與了對(duì)高硝酸鹽脅迫的發(fā)育反應(yīng)，我們的研究表明aba還通過調(diào)節(jié)有限的硝酸鹽供應(yīng)下的nrt2 / nar基因的表達(dá)來促進(jìn)硝酸鹽吸收的優(yōu)化，為改良提供了新的目標(biāo)作物中硝酸鹽的吸收。
不同處理下，小麥根系分生區(qū)、伸長(zhǎng)區(qū)、成熟區(qū)no3-流對(duì)比。
英文摘要
nitrate is the preferred form of nitrogen for most plants, acting both as a nutrient and a signaling molecule. however, the components and regulatory factors governing nitrate uptake in bread wheat (triticum aestivum), one of the world's most important crop species, have remained unclear, largely due to the complexity of its hexaploid genome.
here, based on recently released whole-genome information for bread wheat, the high-affinity nitrate transporter 2 (nrt2) and the nitrate-assimilation-related (nar) gene family are characterized. we show that aba-ge deconjugation is stimulated in bread wheat roots by nitrate resupply following nitrate withdrawal, leading to enhanced root-tissue aba accumulation, and that this enhancement, in turn, affects the expression of root-type nrt2/nar genes. taanr1 is shown to regulate nitrate-mediated aba accumulation by directly activating tabg1, while tawabi5 is involved in aba-mediated no3- induction of nrt2/nar genes.
building on previous evidence establishing aba involvement in the developmental response to high-nitrate stress, our study suggests that aba also contributes to the optimisation of nitrate uptake by regulating the expression of nrt2/nar genes under limited nitrate supply, offering a new target for improvement of nitrate absorption in crops.