anti-ttf1 - rabbit (wrab-1231)使用說明
產(chǎn)品信息
品牌
seven hills bioreagents
貨號
wrab-1231
規(guī)格
100 µl
宿主
兔
反應(yīng)種屬
人、鼠
克隆類型
多克隆
偶聯(lián)物
無
緩沖液
0.01% sodium azide
抗原
重組鼠ttf-1融合6*his蛋白
保存條件
儲存在-20℃，避免反復(fù)凍融
蛋白背景
甲狀腺轉(zhuǎn)錄因子-1（ttf-1）是同源域轉(zhuǎn)錄因子家族的成員，并且在調(diào)節(jié)甲狀腺，肺和腦內(nèi)表達的基因中起作用。
產(chǎn)品使用
實驗應(yīng)用
建議稀釋倍數(shù)
western blot
1: 5000
immunohistochemistry
1:1000 - 1:2000
說明：最佳稀釋倍數(shù)與濃度應(yīng)由研究人員確認
應(yīng)用實例
圖片為成年小鼠肺組織，用6%正常山羊血清預(yù)包埋，并用抗大鼠ttf-1兔血清（1:2000稀釋）染色。通過dab（黑色）觀察ttf-1，并用核快紅（粉紅色）復(fù)染。放大倍數(shù)10倍。
參考文獻
1. zhou l, lim l, costa rh, whitsett ja. 'thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein b, c, and clara cell secretory protein in developing mouse lung.' j histochem cytochem. 1996 oct;44(10):1183-93.
2. stahlman mt, gray me, whitsett ja. 'expression of thyroid transcription factor-1(ttf-1) in fetal and neonatal human lung.' j histochem cytochem. 1996 jul;44(7):673-8.
3. khoor a, whitsett ja, stahlman mt, olson sj, cagle pt. 'utility of surfactant protein b precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' hum pathol. 1999 jun;30(6):695-700.
4. defelice m, silberschmidt d, dilauro r, xu y, wert se, weaver te, bachurski cj, clark jc, whitsett ja. 'ttf-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' j biol chem. 2003 sep 12;278(37):35574-83.
相關(guān)產(chǎn)品
貨號
產(chǎn)品名稱
規(guī)格
品牌
wrab-76694
anti-mature-sp-c - rabbit
100 μl
seven hills bioreagents
wrab-9337
anti-pro-sp-c - rabbit, n-terminal
100 μl
seven hills bioreagents
wrab-3950
anti-ccsp - rabbit
100 μl
seven hills bioreagents
wrab-1236
anti-sox2 - rabbit
100 μl
seven hills bioreagents
wmab-17g524
anti-abca3-mouse(17-h5-22)igm
100 μl
seven hills bioreagents
wrab-1231
anti-ttf1 - rabbit
100 μl
seven hills bioreagents
wrab-55522
anti-pro-sp-b - rabbit
100 μl
seven hills bioreagents
wrab-1200
anti-foxa2 - rabbit
100 μl
seven hills bioreagents
wrab-70565
anti-abca3 - rabbit
100 μl
seven hills bioreagents