睪丸素色譜分析testosterone
conditions
column: ace 5 c18
dimensions: 150 x 0.075 mm
part number: ace-121-1500075
mobile phase:
a: 0.1% formic acid in mecn/0.1% formic acid in h2o (10:90 v/v)
b: 0.1% formic acid in mecn/0.1% formic acid in h2o (90:10 v/v)
gradient:
time(mins)
%b
0
40
5
40
30
95
flow rate: 1 μl/min
temperature: ambient
detection: esi ms/ms
positive ion mode
analyte
1. testosterone