螢火蟲熒光素酶測(cè)定試劑盒2.0是用于轉(zhuǎn)染細(xì)胞裂解物中螢火蟲熒光素酶活性的閃光型測(cè)定。該試劑盒是我們最初的螢火蟲熒光素酶測(cè)定（目錄號(hào)30003）的改進(jìn)版本，具有更好的工作溶液穩(wěn)定性。150次測(cè)定。
艾美捷螢火蟲熒光素酶測(cè)定試劑盒2.0,150次測(cè)定#btm-30085-1組件：
5x 螢火蟲螢光素酶裂解緩沖液
螢火蟲螢光素酶檢測(cè)緩沖液2.0
d-熒光素
螢火蟲熒光素酶測(cè)定試劑盒相關(guān)特征：
高靈敏度和線性閃光型測(cè)定
每次檢測(cè)都添加新鮮的底物，以確保信號(hào)隨時(shí)間推移保持一致
高性價(jià)比
該螢火蟲熒光素酶測(cè)定試劑盒屬性：
化驗(yàn)類型/選項(xiàng)：終點(diǎn)測(cè)定，發(fā)光（閃光型）
檢測(cè)方法/讀數(shù)：光度計(jì)（帶試劑注射器的單管或微量板讀取器）
儲(chǔ)存條件：在-60°c或更低溫度下儲(chǔ)存
firefly luciferase is widely used as a reporter gene to study gene regulation and function and drug screening. since there is no endogenous luciferase activity in mammalian cells or tissues, it is a very sensitive genetic reporter gene. firefly luciferase is a 62000 dalton protein, which is active as a monomer, and does not require subsequent processing of its activity. this enzyme catalyzes the oxidation of atp dependent d-luciferin to oxidized luciferin, producing light emission. the firefly luciferase follows the michaelis menten kinetics, so the maximum light output can be achieved only when the substrate and cofactor are in large excess. when analyzing under these conditions, the light emitted by the reaction is proportional to the number of luciferase molecules.
note: this is a flash type luminescence detection that requires the signal to be measured immediately after adding the working solution to the sample. it requires a single tube luminescent meter or luminescent enzyme reader with a reagent syringe. biotium also offers the steady luc hts firefly detection kit (product number 30028l), which is a homogeneous luminescent detection with a signal half-life of 3-5 hours.
easy to use for consistent results
the firefly luciferase detection kit is designed to simply and effectively quantify the activity of firefly luciferase reporter enzyme in cultured cells, with high sensitivity and linearity. this kit is a new improved substitute for the firefly luciferase test kit (catalog no. 30003). it adopts firefly assay buffer 2.0, which has better working solution stability compared to the original detection buffer.
other commercial luciferase tests require you to dilute all firefly substrates in the test buffer, resulting in a limited shelf life after first use. in biotium's reagent kit, the substrate is provided as a separate stock solution, which is freshly diluted for each test to achieve excellent shelf life and provide consistent signals over time.
the measurement size is based on the 96 hole format. this kit includes sufficient cell lysis buffer to perform the specified number of measurements within the size range of 96 well to 24 well plates.